PDF DatasheetRabbit IGF1 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IGF1 based upon sequence from Rabbit
Host: QP10679 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IGF1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized rbIGF-I in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: IGF-I is biologically active when compared to human IGF-1. The ED50, calculated by the dose -dependent proliferation of human MCF/7 cells is 5 to 25ng/ml in the cell culture mixture dependent on culture conditions. Its activity consists of 30-40 % compared to human IGF-1.
Amino Acid Sequence: MTAGPETLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKP
Purity: Greater than 98.0% as determined by:(a) Gel-filtration chromatography under non denaturing conditions. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized from a concentrated (0.5 mg/ml) solution with 0.02% NaHCO3.
Storage Conditions: Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Rabbit IGF1 General Information | |
|---|---|
| Alternate Names | |
| Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF. | |
| Molecular Weight | |
| 7.6 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q95222 |
| General Description of Recombinant Rabbit IGF1. | |
| Insulin-Like Growth Factor-I Rabbit Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7639 Dalton. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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