PDF DatasheetHuman IGF1 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IGF1 based upon sequence from Human
Host: QP10680 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IGF1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as determined by a cell proliferation assay using murine BALB/C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1×106 units/mg.
Amino Acid Sequence: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC(b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized from a 0.2µm filtered concentrated solution in 20mM PBS, pH 7.0, 130mM NaCl.
Storage Conditions: Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human IGF1 General Information | |
|---|---|
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| Alternate Names | |
| Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF. | |
| Molecular Weight | |
| 7.6 kDa | |
| Chromosomal Location | |
| q23.2 on chromosome 12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IGF1 |
| Entrez Gene ID | 3479 |
| UniProt ID(s) | P01343 |
| COSMIC ID Link(s) | IGF1 |
| KEGG Gene ID(s) | hsa:3479 |
| General Description of Recombinant Human IGF1. | |
| Human Insulin-Like Growth Factor-I produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6kDa. IGF-I is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Selected References
1.Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis. Publication: Journal of Endocrinology (2009) 202, 287297. Link: http://joe.endocrinology-journals.org/content/202/2/287.full.pdfApplication: support spermatogenesis in an androgen-independent manner (Leal et al. 2006)2.Suppression of Anoikis by SKP2 Amplification and Overexpression Promotes Metastasis of Esophageal Squamous Cell Carcinoma .Publication:doi: 10.1158/1541-7786.MCR-08-0092 Mol Cancer Res January 2009 7; 12 Link:http://mcr.aacrjournals.org/content/7/1/12.full3.MECHANISMS OF BIOMATERIAL MEDIATED FIBROTIC RESPONSES AND STRATEGIES TO IMPROVE TISSUE REACTIONS TO BIOMATERIAL IMPLANTS.Publication:THE UNIVERSITY OF TEXAS AT ARLINGTONMay 2010Link:http://dspace.uta.edu/bitstream/handle/10106/4918/Thevenot_uta_2502D_10646.pdf?sequence=1 4. Cancer-Associated Carbonic Anhydrases IX and XII: Effect of Growth Factors on Gene Expression in Human Cancer Cell Lines.Publication: Journal of Cancer Molecules 5(3): 73-78, 2010. Link: http://mupnet.com/JOCM%205(3)%2073-78.pdf
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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