PDF DatasheetFeline IL2 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant IL2 based upon sequence from Feline
Host: QP10727 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of IL2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10727.
Bioactivity Data: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is less or equal to 0.3ng/ml.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSAPASSST KETQQQLEQL LLDLRLLLNG VNNPENPKLS RMLTFKFYVP KKATELTHLQ CLVEELKPLE EVLYLAQSKN FHLNHIKELM SNINVTVLKL KGSETRFTCN YDDETATIVE FLNKWITFCQ SIFSTLT
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: IL2 protein solution (0.25 mg/ml) containing 20mM Tris-HCl(pH 8.0), 0.15M NaCl and 30% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Feline IL2 General Information | |
|---|---|
| Alternate Names | |
| Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2. | |
| Molecular Weight | |
| 17.8 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q07885 |
| General Description of Recombinant Feline IL2. | |
| IL2 Feline Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 157 amino acids (21-154 AA) and having a molecular mass of 17.8kDa. IL2 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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