PDF DatasheetDISC: Porcine Insulin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Insulin based upon sequence from Porcine
Host: QP10733 protein expressed in Native Protein.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Insulin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Insulin Porcine in sterile 0.005N HCl.
Bioactivity Data: >27 units/mg.
Amino Acid Sequence: MALWTRLLPL LALLALWAPA PAQAFVNQHL CGSHLVEALY LVCGERGFFY TPKARREAEN PQAGAVELGG GLGGLQALAL EGPPQKRGIV EQCCTSICSL YQLENYCN
Purity: Greater than 97.0% as determined by SEC-HPLC.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The Insulin Porcine was lyophilized from a concentrated (1 mg/ml) solution with no additives.
Storage Conditions: Lyophilized Insulin Porcine although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Insulin Pig should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Porcine Insulin General Information | |
|---|---|
 |
|
| Molecular Weight | |
| 5.8 kDa | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | INS |
| Entrez Gene ID | 397415 |
| UniProt ID(s) | P01315 |
| COSMIC ID Link(s) | INS |
| KEGG Gene ID(s) | ssc:397415 |
| General Description of Recombinant Porcine Insulin. | |
| Insulin Porcine is a two chain, glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5777 Dalton. The a and b chains are joined by two interchain disulfide bonds. The a chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat. Insulin Porcine is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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