PDF Datasheetother LLO PEST free Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant LLO PEST free based upon sequence from other
Host: QP10774 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of LLO PEST free was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10774.
Bioactivity Data: 7×104 HU/mg. 2mM DTT could be use to reactivate the toxin.
Amino Acid Sequence: MKKIMLVFIT LILISLPIAQ QTEAKDASAF HKEDLISSMA PPTSPPASPK TPIEKKHADE IDKYIQGLDY NKNNVLVYHG DAVTNVPPRK GYKDGNEYIV VEKKKKSINQ NNADIQVVNA ISSLTYPGAL VKANSELVEN QPDVLPVKRD SLTLSIDLPG MTNQDNKIVV KNATKSNVNN AVNTLVERWN EKYAQAYPNV SAKIDYDDEM AYSESQLIAK FGTAFKAVNN SLNVNFGAIS EGKMQEEVIS FKQIYYNVNV NEPTRPSRFF GKAVTKEQLQ ALGVNAENPP AYISSVAYGR QVYLKLSTNS HSTKVKAAFD AAVSGKSVSG DVELTNIIKN SSFKAVIYGG SAKDEVQIID GNLGDLRDIL KKGATFNRET PGVPIAYTTN FLKDNELAVI KNNSEYIETT SKAYTDGKIN IDHSGGYVAQ FNISWDEINY DPEGNEIVQH KNWSENNKSK LAHFTSSIYL PGNARNINVY AKECTGLAWE WWRTVIDDRN LPLVKNRNIS IWGTTLYPKY SNSVDNPIE
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% glycerol and 0.5M NaCl.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant other LLO PEST free General Information | |
|---|---|
| Alternate Names | |
| Listeriolysin-O, LLO, hlyA. | |
| Molecular Weight | |
| 58.8 kDa | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | hlyA |
| UniProt ID(s) | Q9L5B9 |
| COSMIC ID Link(s) | hlyA |
| General Description of Recombinant other LLO PEST free. | |
| Recombinant Listeriolysin O lacking both the signal secretion sequence and the PEST- like sequence. LLO-PEST minus is composed of 471 amino acids, starting from amino acid 60 to amino acid 529, with the addition of a methionine in its NH2-terminus. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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