PDF DatasheetHuman Fragile histidine triad / FHIT Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Fragile histidine triad / FHIT based upon sequence from Human
Host: QP1078 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Fragile histidine triad / FHIT, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP1078.
Bioactivity Data: Untested
Full Length? Full Length
Expression Region: Ser2 – Gln147
Amino Acid Sequence: SFRFGQHLIK PSVVFLKTEL SFALVNRKPV VPGHVLVCPL RPVERFHDLR PDEVADLFQT TQRVGTVVEK HFHGTSLTFS MQDGPEAGQT VKHVHVHVLP RKAGDFHRND SIYEELQKHD KEDFPASWRS EEEMAAEAAA LRVYFQ
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human Fragile histidine triad / FHIT Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Fragile histidine triad / FHIT Protein General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| Gene Symbol | FHIT |
| Entrez Gene ID | 2272 |
| Ensemble Gene ID | ENSG00000189283 |
| RefSeq Protein Accession(s) | NP_001159715.1 |
| RefSeq mRNA Accession(s) | NM_001166243.2, NM_001320899.1, NM_001320900.1, NM_002012.3 |
| UniProt ID(s) | P49789 |
| UniGene ID(s) | Hs.655995 |
| HGNC ID(s) | HGNC:3701 |
| COSMIC ID Link(s) | FHIT |
| KEGG Gene ID(s) | hsa:2272 |
| PharmGKB ID(s) | PA28140 |
| General Description of Recombinant Human Fragile histidine triad / FHIT Protein. | |
| Cleaves P(1)-P(3)-bis(5′-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5′-adenosyl) tetraphosphate (Ap4A), but has extrely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5′-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca2+ transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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