PDF DatasheetHuman MMP-2 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant MMP-2 based upon sequence from Human
Host: QP10790 protein expressed in HEK 293.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of MMP-2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10790.
Bioactivity Data: The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RND,Catalog # ES001). , The specific activity is > 1,000 pmoles/min/µg. Recombinant Human MMP-2 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA). Activation Protocol:1. Dilute MMP2 to 100µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5. 2. Activate MMP2 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO. 3. Incubate at 37°C for 1 hour.
Amino Acid Sequence: MEALMARGAL TGPLRALCLL GCLLSHAAAA PSPIIKFPGD VAPKTDKELA VQYLNTFYGC PKESCNLFVL KDTLKKMQKF FGLPQTGDLD QNTIETMRKP RCGNPDVANY NFFPRKPKWD KNQITYRIIG YTPDLDPETV DDAFARAFQV WSDVTPLRFS RIHDGEADIM INFGRWEHGD GYPFDGKDGL LAHAFAPGTG VGGDSHFDDD ELWTLGEGQV VRVKYGNADG EYCKFPFLFN GKEYNSCTDT GRSDGFLWCS TTYNFEKDGK YGFCPHEALF TMGGNAEGQP CKFPFRFQGT SYDSCTTEGR TDGYRWCGTT EDYDRDKKYG FCPETAMSTV GGNSEGAPCV FPFTFLGNKY ESCTSAGRSD GKMWCATTAN YDDDRKWGFC PDQGYSLFLV AAHEFGHAMG LEHSQDPGAL MAPIYTYTKN FRLSQDDIKG IQELYGASPD IDLGTGPTPT LGPVTPEICK QDIVFDGIAQ IRGEIFFFKD RFIWRTVTPR DKPMGPLLVA TFWPELPEKI DAVYEAPQEE KAVFFAGNEY WIYSASTLER GYPKPLTSLG LPPDVQRVDA AFNWSKNKKT YIFAGDKFWR YNEVKKKMDP GFPKLIADAW NAIPDNLDAV VDLQGGGHSY FFKGAYYLKL ENQSLKSVKF GSIKSDWLGC
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The MMP-2 is supplied as a 0.2µm filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij 35, pH 7.4.
Storage Conditions: Store MMP-2 at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: The MMP-2 is supplied as a sterile Filtered colorless solution.
| Recombinant Human MMP-2 General Information | |
|---|---|
| Alternate Names | |
| 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, TBE-1, MMP2, CLG4A, CLG4, MONA, MMP-II. | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P08253 |
| General Description of Recombinant Human MMP-2. | |
| Human MMP-2 produced in HEK293 cells is a proform of the Human MMP-2 (Ala30-Cys660) and fused with a polyhistidine tag at the C-terminus, having an MW of 71kDa. MMP-2 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Selected References
MMP-2 regulates human platelet activation by interacting withintegrin aIIbb 3.Publication:To cite this article: Choi W-S, Jeon O-H, Kim H-H, Kim D-S. MMP-2 regulates human platelet activation by interacting with integrin aIIbb3.J Thromb Haemost 2008; 6: 51723.Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1538-7836.2007.02871.x/pdf
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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