PDF DatasheetHuman PIN1 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant PIN1 based upon sequence from Human
Host: QP10820 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of PIN1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10820.
Bioactivity Data: Specific activity is > 330 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-HCl pH8.0 using chymotrypsin.
Amino Acid Sequence: MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The PIN1 protein solution (1 mg/ml) containing 20mM Tris-HCl buffer (pH 7.5) 0.1M NaCl, 5mM DTT & 20% Glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human PIN1 General Information | |
|---|---|
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| Alternate Names | |
| Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC 5.2. 1.8, Rotamase Pin1, PPIase Pin1, DOD, UBL5, PIN1, PPIase. | |
| Molecular Weight | |
| 18.2 kDa | |
| Chromosomal Location | |
| p13.2 on chromosome 19 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PIN1 |
| Entrez Gene ID | 5300 |
| UniProt ID(s) | Q13526 |
| COSMIC ID Link(s) | PIN1 |
| KEGG Gene ID(s) | hsa:5300 |
| General Description of Recombinant Human PIN1. | |
| Human PPIase produced in E. Coli is a single, non-glycosylated polypeptide chain containing 163 amino acids & having a molecular mass of 18.2 kDa. The PIN1 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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