PDF DatasheetMouse PKM2 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant PKM2 based upon sequence from Mouse
Host: QP10825 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PKM2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10825.
Bioactivity Data: Specific activity: > 500 units/mg. One unit will convert 1 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37°C.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPKPHSE AGTAFIQTQQ LHAAMADTFL EHMCRLDIDS APITARNTGI ICTIGPASRS VEMLKEMIKS GMNVARLNFS HGTHEYHAET IKNVREATES FASDPILYRP VAVALDTKGP EIRTGLIKGS GTAEVELKKG ATLKITLDNA YMEKCDENIL WLDYKNICKV VEVGSKIYVD DGLISLQVKE KGADFLVTEV ENGGSLGSKK GVNLPGAAVD LPAVSEKDIQ DLKFGVEQDV DMVFASFIRK AADVHEVRKV LGEKGKNIKI ISKIENHEGV RRFDEILEAS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC NRAGKPVICA TQMLESMIKK PRPTRAEGSD VANAVLDGAD CIMLSGETAK GDYPLEAVRM QHLIAREAEA AIYHLQLFEE LRRLAPITSD PTEAAAVGAV EASFKCCSGA IIVLTKSGRS AHQVARYRPR APIIAVTRNP QTARQAHLYR GIFPVLCKDA VLNAWAEDVD LRVNLAMDVG KARGFFKKGD VVIVLTGWRP GSGFTNTMRV VPVP
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: PKM2 protein solution (0.5 mg/ml) containing 20mM Tris-HCl (pH 8.5), 0.2M NaCl, 1mM DTT and 30% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Mouse PKM2 General Information | |
|---|---|
| Alternate Names | |
| Pyruvate kinase isozymes M1/M2, EC 2.7. 1.40, Pyruvate kinase muscle isozyme, Pyruvate kinase 2/3, Cytosolic thyroid hormone-binding protein, CTHBP, THBP1, M2PK, PKM2, PK3, PK2, PKM, TCB, OIP3, MGC3932, Tumor Type M2 Pyruvate Kinase. | |
| Molecular Weight | |
| 60.2 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P52480 |
| General Description of Recombinant Mouse PKM2. | |
| Mouse PKM2 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 554 amino acids (1-531 AA) and having a molecular mass of 60.2kDa. PKM2 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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