PDF DatasheetHuman PPIL3 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant PPIL3 based upon sequence from Human
Host: QP10833 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PPIL3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10833.
Bioactivity Data: Specific activity is > 280 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSVTLHTDVG DIKIEVFCER TPKTCENFLA LCASNYYNGC IFHRNIKGFM VQTGDPTGTG RGGNSIWGKK FEDEYSEYLK HNVRGVVSMA NNGPNTNGSQ FFITYGKQPH LDMKYTVFGK VIDGLETLDE LEKLPVNEKT YRPLNDVHIK DITIHANPFA Q
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: PPIL3 protein solution (1 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human PPIL3 General Information | |
|---|---|
 |
|
| Alternate Names | |
| Peptidyl-prolyl cis-trans isomerase-like 3, PPIase, Cyclophilin J, CyPJ, Cyclophilin-like protein PPIL3, Rotamase PPIL3, PPIL3. | |
| Molecular Weight | |
| 20.3 kDa | |
| Chromosomal Location | |
| q33.1 on chromosome 2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PPIL3 |
| Entrez Gene ID | 53938 |
| UniProt ID(s) | Q9H2H8 |
| COSMIC ID Link(s) | PPIL3 |
| KEGG Gene ID(s) | hsa:53938 |
| General Description of Recombinant Human PPIL3. | |
| Human PPIL3 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 181 amino acids (1-161 AA ) and having a molecular mass of 20.3kDa. PPIL3 is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.