PDF DatasheetHuman PRDX5 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant PRDX5 based upon sequence from Human
Host: QP10841 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of PRDX5 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10841.
Bioactivity Data: The specific activity was found to be approximately 117-136 pmole/min/µg. The enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX5 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25°C for 1 minute.
Amino Acid Sequence: MAPIKVGDAI PAVEVFEGEP GNKVNLAELF KGKKGVLFGV PGAFTPGCSK THLPGFVEQA EALKAKGVQV VACLSVNDAF VTGEWGRAHK AEGKVRLLAD PTGAFGKETD LLLDDSLVSI FGNRRLKRFS MVVQDGIVKA LNVEPDGTGL TCSLAPNIIS QL
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The PRDX5 solution contains 20mM HEPES buffer (pH 7.4).
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human PRDX5 General Information | |
|---|---|
| Alternate Names | |
| Peroxiredoxin-5 mitochondrial, Prx-V, Peroxisomal antioxidant enzyme, Thioredoxin reductase, Thioredoxin peroxidase PMP20, Antioxidant enzyme B166, TPx type VI, Liver tissue 2D-page spot 71B, Alu corepressor 1, PLP, ACR1, B166, PRXV, PMP20, PRDX6, SBBI10, AOEB166, MGC117264, MGC142283, MGC142285, PRDX5. | |
| Molecular Weight | |
| 17 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P30044 |
| General Description of Recombinant Human PRDX5. | |
| Human PRDX5 produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 162 amino acids (53-214 AA ) and having a molecular mass of 17 kDa. The PRDX5 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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