PDF DatasheetHuman PRDX6 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant PRDX6 based upon sequence from Human
Host: QP10842 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PRDX6 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10842.
Bioactivity Data: The specific activity was found to be approximately 95-120 pmole/min/µg. The enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX6 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25°C for 1 minute.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The Peroxiredoxin-6 solution contains 20mM Tris-HCl buffer (pH 8.0) and 20% Glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human PRDX6 General Information | |
|---|---|
| Alternate Names | |
| Peroxiredoxin-6, Antioxidant protein 2, 1-Cys peroxiredoxin, Acidic calcium-independent phospholipase A2, Non-selenium glutathione peroxidase, 24 kDa protein, Liver 2D page spot 40, Red blood cells page spot 12, 1-Cys PRX, aiPLA2, NSGPx, PRDX6, AOP2, KIAA0106, PRX, p29, 1-Cys, MGC46173. | |
| Molecular Weight | |
| 27.1 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P30041 |
| General Description of Recombinant Human PRDX6. | |
| Human Peroxiredoxin- 6 fused with a 20 amino acid His tag at N-terminus produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 244 amino acids (1-224 AA ) and having a molecular mass of 27.1kDa. The Peroxiredoxin-6 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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