PDF DatasheetRainbow Trout PRL R Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant PRL R based upon sequence from Rainbow Trout
Host: QP10844 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of PRL R was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized PRL-R in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Activity is determined by the dose-dependant inhibition of Prolactin-stimuled proliferation of Nb2 cells and by high affinity binding of oPLR and other lactogenic hormones.
Amino Acid Sequence: MMWRDVGVTL ILSLILPVVA QGARHTPPGK PKLTSCRSPD KETFTCWWEA GSDGGRPTTY SLFYHKENSE TVYECPDYHT AGANSCFFNK NETSIWVNYN ITVVATNALG NNFSDPVDVD VVYIVQPHTP ENVTVGVLED EDGPFLRVSW EPPRKADTRS GWITLIYELR VKLEEAEEWE EHRAGQQKMF NNFSLHSGGV YMVQVRCKPD HGFWSEWSTA SYVKVPDYIP RERSMWILIA VFSAFIFLIL TWIITLNRSS VKHCLLPPVP GPKIKGFDQQ LLKKGKPEEV FNSLVVQSFP PRSTDYEDLL VEYLEVYVNE KQELMPEGKD LRDVGCLESK SQSDNDSGRG SCDSHTLLME KCGGVESKDE SSYEEPELLQ GQAGSWERLE RGDSQVVDTP DSSDARVKTW PLVFSPSIHG SSDNHHYGTL EMPKQHSTSE ITYSVPDHLF PTSTSFPHHH HPEYRDSLGE YSEYRHSRSP STGVLYHWEA QHHPQAHSNF NIRNLERQKE ATGFGLPPSR SMEYVEVQKV NQNQLVLKPL SSHGRGPSQV QFGRAVVDYS KVNGVNNDNV LLLQRQREMA EAGQYCDYRE KEGAEAERYP TQQQGKTTKD GPVATQLQES TCLTTSGYVD TVPTMPTF
Purity: Greater than 97.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Rainbow Trout PRL R Protein: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 2.48 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of PRLr-ECD as a Reference Standard.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045mM NaHCO3.
Storage Conditions: Lyophilized PRL-R although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prolactin Receptor should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Rainbow Trout PRL R General Information | |
|---|---|
| Alternate Names | |
| PRL-R. | |
| Molecular Weight | |
| 24 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9DE08 |
| General Description of Recombinant Rainbow Trout PRL R. | |
| Prolactin Receptor Rainbow Trout Extra Cellular Domain Recombinant ?produced in E. Coli is a non-glycosylated, Polypeptide chain containing 210 amino acids and having a molecular mass of 24034 Dalton. The Prolactin Receptor is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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