PDF DatasheetMouse TGFB3 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant TGFB3 based upon sequence from Mouse
Host: QP10876 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of TGFB3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10876.
Bioactivity Data: The activity is determined by the ability to induce chondrogenic differentiation.
Monomer or Dimer: Dimer
Amino Acid Sequence: ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS
Purity: Greater than 96.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Concentration of Mouse TGFB3 Protein: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TGF-b 3 as a Reference Standard.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The Mouse TGFB3 protein solution contains 20% Ethanol and 0.12% Acetic acid.
Storage Conditions: Mouse TGF-beta 3 although stable at room temperature for 3 weeks, should be stored at 4°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Mouse TGFB3 General Information | |
|---|---|
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| Alternate Names | |
| Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3. | |
| Molecular Weight | |
| 25.5 kDa | |
| Chromosomal Location | |
| D2 on chromosome 12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Tgfb3 |
| Entrez Gene ID | 21809 |
| UniProt ID(s) | Q99K17 |
| COSMIC ID Link(s) | Tgfb3 |
| KEGG Gene ID(s) | mmu:21809 |
| General Description of Recombinant Mouse TGFB3. | |
| Mouse TGF-b3 produced in E. Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 112 amino acids and having a molecular mass of 25.5kDa. The TGF-b 3 is purified by standard chromatographic techniques. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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