PDF DatasheetHuman TPI1 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant TPI1 based upon sequence from Human
Host: QP10891 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of TPI1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10891.
Bioactivity Data: Specific activity is > 3000 units/mg, in which one unit will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The TPI1 protein solution (0.5 mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human TPI1 General Information | |
|---|---|
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| Alternate Names | |
| TPI, TIM, Triosephosphate Isomerase 1. | |
| Molecular Weight | |
| 28.8 kDa | |
| Chromosomal Location | |
| p13.31 on chromosome 12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TPI1 |
| Entrez Gene ID | 7167 |
| UniProt ID(s) | P60174 |
| COSMIC ID Link(s) | TPI1 |
| KEGG Gene ID(s) | hsa:7167 |
| General Description of Recombinant Human TPI1. | |
| TPI1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 269 amino acids (1-249AA ) and having a molecular mass of 28.8kDa. TPI1 is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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