PDF DatasheetE. Coli TRXR Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant TRXR based upon sequence from E. Coli
Host: QP10894 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of TRXR was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10894.
Bioactivity Data: Specific activity is 4-5 units/ml, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.
Amino Acid Sequence: MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: TRXR E. Coli solution containing 20mM Tris HCl pH 8.0, 1mM DTT, and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant E. Coli TRXR General Information | |
|---|---|
| Alternate Names | |
| TRXB, TRXR, Thioredoxin Reductase. | |
| Molecular Weight | |
| 34.6 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P0A9P4 |
| General Description of Recombinant E. Coli TRXR. | |
| TRXR E. coli Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 321 amino acids (1-321 AA ) and having a molecular mass of 34.6 kDa. TRXR protein is purified by standard chromatography. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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