PDF DatasheetHuman Trypsin-1 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Trypsin-1 based upon sequence from Human
Host: QP10898 protein expressed in CHO.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Trypsin-1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10898.
Bioactivity Data: 1 unit hydrolyzes 1 µmole of Na-Benzoyl-D,L-arginine 4-nitroanilide (BANI) per minute at 37°C, pH 8.0, in the presence of 10mM calcium. The specific activity is >1,000 Units per mg protein as measured under the described conditions.
Amino Acid Sequence: IVGGYNCEEN SVPYQVSLNS GYHFCGGSLI NEQWVVSAGH CYKSRIQVRL GEHNIEVLEG NEQFINAAKI IRHPQYDRKT LNNDIMLIKL SSRAVINARV STISLPTAPP ATGTKCLISG WGNTASSGAD YPDELQCLDA PVLSQAKCEA SYPGKITSNM FCVGFLEGGK DSCQGDSGGP VVCNGQLQGV VSWGDGCAQK NKPGVYTKVY NYVKWIKNTI AANS
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The Trypsin-1 filtered 0.22um solution contains 20mM TRIS-HCl buffer pH 7.2.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human Trypsin-1 General Information | |
|---|---|
| Alternate Names | |
| Protease Serine 1 (Trypsin 1), TRY1, Cationic Trypsinogen, TRP1, TRYP1, TRY4, beta-trypsin, Digestive Zymogen, Nonfunctional Trypsin 1, Trypsinogen 1, Trypsinogen A, EC 3.4. 21.4. | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P07477 |
| General Description of Recombinant Human Trypsin-1. | |
| Human Trypsin-1 produced in CHO cells is a 24.1kDa single polypeptide chain consisting of 224 amino acids. | |
Limitations and Performance Guarantee
enQuire Bio’s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used for any other purpose. This product is guaranteed to work for a period of one year when stored at -70°C or colder.
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