PDF DatasheetMouse UCHL3 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant UCHL3 based upon sequence from Mouse
Host: QP10905 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of UCHL3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes: Please contact us for application specific information for QP10905.
Bioactivity Data: Specific activity is > 9,000 pmol/min/mg, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37°C.
Amino Acid Sequence: MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMEPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERAKFLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGKTSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA LEHHHHHH
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: UCHL3 protein solution (0.5 mg/ml) containing Phosphate buffered saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Mouse UCHL3 General Information | |
|---|---|
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| Alternate Names | |
| Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, UCHL3, Ubiquitin Carboxyl-Terminal Esterase L3, Ubiquitin thioesterase L3, Uchl3, Ubiquitin carboxyl-terminal esterase L3. | |
| Molecular Weight | |
| 27.2 kDa | |
| Chromosomal Location | |
| E2.3 on chromosome 14 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Uchl3 |
| Entrez Gene ID | 50933 |
| UniProt ID(s) | Q9JKB1 |
| COSMIC ID Link(s) | Uchl3 |
| KEGG Gene ID(s) | mmu:50933 |
| General Description of Recombinant Mouse UCHL3. | |
| Mouse UCHL3 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 238 amino acids (1-230) and having a molecular mass of 27.2kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). UCHL3 is fused to 8 amino acid His-Tag at C-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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