PDF Datasheetother Ag85A Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Ag85A based upon sequence from other
Host: QP10976 protein expressed in Baculovirus.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of Ag85A was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP10976.
Bioactivity Data: Untested
Amino Acid Sequence: ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The Ag85A solution (0.5 mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant other Ag85A General Information | |
|---|---|
| Alternate Names | |
| Ronectin-binding protein A, Mycolyl transferase 85A. | |
| Molecular Weight | |
| 32.8 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P0A4V2 |
| General Description of Recombinant other Ag85A. | |
| Recombinant Mycobacterium tuberculosis Ag85A (43-338 AA) produced in Hi-5 cells is a single, glycosylated polypeptide chain having a molecular mass of 32.8kDa (305 a. a in total). Antigen 85A is fused to a His tag at C-terminus and purified by conventional chromatographic techniques. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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