PDF DatasheetHuman AK5 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant AK5 based upon sequence from Human
Host: QP10992 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of AK5 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP10992.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMNTNDA KEYMARREIP QLFESLLNGL MCSKPEDPVE YLESCLQKVK ELGGCDKVKW DTFVSQEKKT LPPLNGGQSR RSFLRNVMPG NSNFPYRRYD RLPPIHQFSI ESDTDLSETA ELIEEYEVFD PTRPRPKIIL VIGGPGSGKG TQSLKIAERY GFQYISVGEL LRKKIHSTSS NRKWSLIAKI ITTGELAPQE TTITEIKQKL MQIPDEEGIV IDGFPRDVAQ ALSFEDQICT PDLVVFLACA NQRLKERLLK RAEQQGRPDD NVKATQRRLM NFKQNAAPLV KYFQEKGLIM TFDADRDEDE VFYDISMAVD NKLFPNKEAA AGSSDLDPSM ILDTGEIIDT GSDYEDQGDD QLNVFGEDTM GGFMEDLRKC KIIFIIGGPG SGKGTQCEKL VEKYGFTHLS TGELLREELA SESERSKLIR DIMERGDLVP SGIVLELLKE AMVASLGDTR GFLIDGYPRE VKQGEEFGRR IGDPQLVICM DCSADTMTNR LLQRSRSSLP VDDTTKTIAK RLEAYYRASI PVIAYYETKT QLHKINAEGT PEDVFLQLCT AIDSIF
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: AK5 protein solution (0.25 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 20% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human AK5 General Information | |
|---|---|
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| Alternate Names | |
| Adenylate kinase isoenzyme 5, AK 5, ATP-AMP transphosphorylase 5, AK5, AK6. | |
| Molecular Weight | |
| 65.9 kDa | |
| Chromosomal Location | |
| p31.1 on chromosome 1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | AK5 |
| Entrez Gene ID | 26289 |
| UniProt ID(s) | Q9Y6K8 |
| COSMIC ID Link(s) | AK5 |
| KEGG Gene ID(s) | hsa:26289 |
| General Description of Recombinant Human AK5. | |
| Human AK5 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 586 amino acids (1-562 AA ) and having a molecular mass of 65.9kDa. AK5 is fused to a 24 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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