PDF DatasheetHuman ASF1B Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ASF1B based upon sequence from Human
Host: QP11084 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of ASF1B was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11084.
Bioactivity Data: Untested
Amino Acid Sequence: MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTPIKGLGLPGCI PGLLPENSMD CILEHHHHHH
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: ASF1B protein solution (1 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.15M NaCl and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human ASF1B General Information | |
|---|---|
| Alternate Names | |
| Histone chaperone ASF1B, Anti-silencing function protein 1 homolog B, hAsf1, hAsf1b, CCG1-interacting factor A-II, CIA-II, hCIA-II, ASF1B. | |
| Molecular Weight | |
| 23.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9NVP2 |
| General Description of Recombinant Human ASF1B. | |
| Human ASF1B produced in E. Coli is a single, non-glycosylated polypeptide chain containing 210 amino acids (1-202 AA) and having a molecular mass of 23.4kDa. ASF1B is fused to an 8 amino acid His-tag at C-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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