PDF DatasheetHuman ATP5F1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ATP5F1 based upon sequence from Human
Host: QP11108 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of ATP5F1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11108.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSLILYALS KEIYVISAET FTALSVLGVM VYGIKKYGPF VADFADKLNE QKLAQLEEAK QASIQHIQNA IDTEKSQQAL VQKRHYLFDV QRNNIAMALE VTYRERLYRV YKEVKNRLDY HISVQNMMRR KEQEHMINWV EKHVVQSIST QQEKETIAKC IADLKLLAKK AQAQPVM
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The ATP5F1 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human ATP5F1 General Information | |
|---|---|
| Alternate Names | |
| ATP Synthase Proton-Transporting Mitochondrial F(0) Complex Subunit B1, ATP Synthase H+ Transporting, Mitochondrial Fo Complex Subunit B Isoform 1, ATPase Subunit B, ATP Synthase B Chain Mitochondrial, Cell Proliferation-Inducing Protein 47, PIG47. | |
| Molecular Weight | |
| 22.6 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P24539 |
| General Description of Recombinant Human ATP5F1. | |
| Human ATP5F1 produced in E. coli is a single, non-glycosylated polypeptide chain containing 197 amino acids (83-256) and having a molecular mass of 22.6 kDa. ATP5F1 is fused to a 23 amino acid His-tag at N-terminus. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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