PDF DatasheetHuman AUH Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant AUH based upon sequence from Human
Host: QP11111 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of AUH was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11111.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSEMKTEDE LRVRHLEEEN RGIVVLGINR AYGKNSLSKN LIKMLSKAVD ALKSDKKVRT IIIRSEVPGI FCAGADLKER AKMSSSEVGP FVSKIRAVIN DIANLPVPTI AAIDGLALGG GLELALACDI RVAASSAKMG LVETKLAIIP GGGGTQRLPR AIGMSLAKEL IFSARVLDGK EAKAVGLISH VLEQNQEGDA AYRKALDLAR EFLPQGPVAM RVAKLAINQG MEVDLVTGLA IEEACYAQTI PTKDRLEGLL AFKEKRPPRY KGE
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The AUH solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human AUH General Information | |
|---|---|
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| Alternate Names | |
| Methylglutaconyl-CoA hydratase, mitochondrial, AU-specific RNA-binding enoyl-CoA hydratase, AU-binding protein/enoyl-CoA hydratase, AUH. | |
| Molecular Weight | |
| 31.4 kDa | |
| Chromosomal Location | |
| q22.31 on chromosome 9 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | AUH |
| Entrez Gene ID | 549 |
| UniProt ID(s) | Q13825 |
| COSMIC ID Link(s) | AUH |
| KEGG Gene ID(s) | hsa:549 |
| General Description of Recombinant Human AUH. | |
| Human AUH fused with a 21 amino acid His tag at N-terminus produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 293 amino acids (68-339 AA ) and having a molecular mass of 31.4kDa. The AUH is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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