PDF DatasheetHuman BCAM Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant BCAM based upon sequence from Human
Host: QP11132 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of BCAM was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11132.
Bioactivity Data: Untested
Amino Acid Sequence: EVRLSVPPLV EVMRGKSVIL DCTPTGTHDH YMLEWFLTDR SGARPRLASA EMQGSELQVT MHDTRGRSPP YQLDSQGRLV LAEAQVGDER DYVCVVRAGA AGTAEATARL NVFAKPEATE VSPNKGTLSV MEDSAQEIAT CNSRNGNPAP KITWYRNGQR LEVPVEMNPE GYMTSRTVRE ASGLLSLTST LYLRLRKDDR DASFHCAAHY SLPEGRHGRL DSPTFHLTLH YPTEHVQFWV GSPSTPAGWV REGDTVQLLC RGDGSPSPEY TLFRLQDEQE EVLNVNLEGN LTLEGVTRGQ SGTYGCRVED YDAADDVQLS KTLELRVAYL DPLELSEGKV LSLPLNSSAV VNCSVHGLPT PALRWTKDST PLGDGPMLSL SSITFDSNGT YVCEASLPTV PVLSRTQNFT LLVQGSPELK TAEIEPKADG SWREGDEVTL ICSARGHPDP KLSWSQLGGS PAEPIPGRQG WVSSSLTLKV TSALSRDGIS CEASNPHGNK RHVFHFGTVS PQTSQAVEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: BCAM protein solution (0.5 mg/ml) containing Phosphate Buffered Saline (pH 7.4), and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human BCAM General Information | |
|---|---|
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| Alternate Names | |
| Basal cell adhesion molecule isoform 1, BCAM, AU, CD239, LU, MSK19, Auberger B antigen, B-CAM cell surface glycoprotein, F8/G253 antigen, Lutheran antigen, Lutheran blood group glycoprotein, CD_antigen: CD239, LU, MSK19. | |
| Molecular Weight | |
| 83.2 kDa | |
| Chromosomal Location | |
| q13.32 on chromosome 19 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | BCAM |
| Entrez Gene ID | 4059 |
| UniProt ID(s) | P50895 |
| COSMIC ID Link(s) | BCAM |
| KEGG Gene ID(s) | hsa:4059 |
| General Description of Recombinant Human BCAM. | |
| BCAM produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 755 amino acids (32-547AA ) and having a molecular mass of 83.2kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). BCAM is expressed with a 239 amino acid hIgG-His tag at C-Terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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