PDF DatasheetHuman BCL2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant BCL2 based upon sequence from Human
Host: QP11142 protein expressed in E. Coli.
Available Tags: Untagged, His
Protein Construction: A cDNA sequence encoding the sequence of BCL2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11142.
Bioactivity Data: Untested
Monomer or Dimer: Dimer
Amino Acid Sequence: AHAGRTGYDN REIVMKYIHY KLSQRGYEWD AGDVGAAPPG AAPAPGIFSS QPGHTPHPAA SRDPVARTSP LQTPAAPGAA AGPALSPVPP VVHLTLRQAG DDFSRRYRRD FAEMSSQLHL TPFTARGRFA TVVEELFRDG VNWGRIVAFF EFGGVMCVES VNREMSPLVD NIALWMTEYL NRHLHTWIQD NGGWDAFVEL YGPSMRPLFD
Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: BCL2 is a 0.2µm filtered concentrated solution which contains 25 mM Tris-HCl, pH 8.0, 100mM NaCl, 1 mM DTT, 30 % Glycerol, and 0.01%Tween-80.
Storage Conditions: BCL2 although stable at room temperature for 3 weeks, should be stored at 4°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human BCL2 General Information | |
|---|---|
| Alternate Names | |
| Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2. | |
| Molecular Weight | |
| 23.2 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P10415 |
| General Description of Recombinant Human BCL2. | |
| Recombinant Human BCL2 produced in E. coli cells is a non-glycosylated, homodimeric protein containing 210 amino acid chain and having a molecular mass of 23.2kDa. The Human BCL2 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. The products may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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