PDF DatasheetHuman BRAF Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant BRAF based upon sequence from Human
Host: QP11200 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of BRAF was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11200.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFSEDRN RMKTLGRRDS SDDWEIPDGQ ITVGQRIGSG SFGTVYKGKW HGDVAVKMLN VTAPTPQQLQ AFKNEVGVLR KTRHVNILLF MGYSTKPQLA IVTQWCEGSS LYHHLHIIET KFEMIKLIDI ARQTAQGMDY LHAKSIIHRD LKSNNIFLHE DLTVKIGDFG LATVKSRWSG SHQFEQLSGS ILWMAPEVIR MQDKNPYSFQ SDVYAFGIVL YELMTGQLPY SNINNRDQII FMVGRGYLSP DLSKVRSNCP KAMKRLMAEC LKKKRDERPL FPQILASIEL LARSLPKIHR SASEPSLNRA GFQTEDFSLY ACASPKTPIQ AGGYGAFPVH
Purity: Greater than 80.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: BRAF protein solution (0.25 mg/ml) containing 20mM Tris-HCl (pH 8.0) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human BRAF General Information | |
|---|---|
| Alternate Names | |
| B-Raf Proto-Oncogene, Serine/Threonine Kinase, V-Raf Murine Sarcoma Viral Oncogene Homolog B1, V-Raf Murine Sarcoma Viral Oncogene Homolog B, Proto-Oncogene B-Raf, BRAF1, RAFB1, NS7, B-Raf Proto-Oncogene Serine/Threonine-Protein Kinase (P94), Murine Sarcoma Viral (V-Raf) Oncogene Homolog B1, Serine/Threonine-Protein Kinase B-Raf, 94 KDa B-Raf Protein, EC 2.7. 11.1, B-RAF1, P94, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1. | |
| Molecular Weight | |
| 40.6 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P15056 |
| General Description of Recombinant Human BRAF. | |
| Human BRAF produced in E. Coli is a single, non-glycosylated polypeptide chain containing 360 amino acids (432-766AA) and having a molecular mass of 40.6kDa. BRAF is fused to a 25 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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