PDF DatasheetHuman HIF-1 alpha / HIF1A Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HIF-1 alpha / HIF1A based upon sequence from Human
Host: QP1121 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) HIF-1 alpha / HIF1A, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Recommended Applications: Immunogen, Protein Standard, Cell culture, or Other Cell Biology Applications.
Application Notes: Please contact us for application specific information for QP1121.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Gln579 – Asn826
Amino Acid Sequence: QLSPLESSSA SPESASPQST VTVFQQTQIQ EPTANATTTT ATTDELKTVT KDRMEDIKIL IASPSPTHIH KETTSATSSP YRDTQSRTAS PNRAGKGVIE QTEKSHPRSP NVLSVALSQR TTVPEEELNP KILALQNAQR KRKMEHDGSL FQAVGIGTLL QQPDDHAATT SLSWKRVKGC KSSEQNGMEQ KTIILIPSDL ACRLLGQSMD ESGLPQLTSY DCEVNAPIQG SRNLLQGEEL LRALDQVN
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: Concentrated protein in liquid format. Reconstitution is not necessary.
Concentration of Human HIF-1 alpha / HIF1A Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human HIF-1 alpha / HIF1A Protein General Information | |
|---|---|
| Alternate Names | |
| HIF-1 alpha, HIF1A, HIF-1-alpha, HIF1-ALPHA, HIF1, HIF-1alpha, PASD8, MOP1, HIF-1A, bHLHe78 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | HIF1A |
| Entrez Gene ID | 3091 |
| Ensemble Gene ID | ENSG00000100644 |
| RefSeq Protein Accession(s) | NP_001230013.1 |
| RefSeq mRNA Accession(s) | NM_001243084.1, NM_001530.3, NM_181054.2 |
| UniProt ID(s) | Q16665 |
| UniGene ID(s) | Hs.597216 |
| HGNC ID(s) | HGNC:4910 |
| COSMIC ID Link(s) | HIF1A |
| KEGG Gene ID(s) | hsa:3091 |
| PharmGKB ID(s) | PA29283 |
| General Description of Recombinant Human HIF-1 alpha / HIF1A Protein. | |
| Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in bryonic vascularization, tumor angiogenesis and pathophysiology of ischic disease. Binds to core DNA sequence 5′-[AG]CGTG-3′ within the hypoxia response elent (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX ses to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.