PDF DatasheetHuman BUB3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant BUB3 based upon sequence from Human
Host: QP11212 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of BUB3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11212.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTGSNEF KLNQPPEDGI SSVKFSPNTS QFLLVSSWDT SVRLYDVPAN SMRLKYQHTG AVLDCAFYDP THAWSGGLDH QLKMHDLNTD QENLVGTHDA PIRCVEYCPE VNVMVTGSWD QTVKLWDPRT PCNAGTFSQP EKVYTLSVSG DRLIVGTAGR RVLVWDLRNM GYVQQRRESS LKYQTRCIRA FPNKQGYVLS SIEGRVAVEY LDPSPEVQKK KYAFKCHRLK ENNIEQIYPV NAISFHNIHN TFATGGSDGF VNIWDPFNKK RLCQFHRYPT SIASLAFSND GTTLAIASSY MYEMDDTEHP EDGIFIRQVT DAETKPKSPC T
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: BUB3 protein solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human BUB3 General Information | |
|---|---|
| Alternate Names | |
| BUB3L, hBUB3, Mitotic checkpoint protein BUB3. | |
| Molecular Weight | |
| 39.5 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | O43684 |
| General Description of Recombinant Human BUB3. | |
| Human BUB3 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 351 amino acids (1-328 AA ) and having a molecular mass of 39.5kDa. BUB3 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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