PDF DatasheetHuman CD247 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CD247 based upon sequence from Human
Host: QP11317 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CD247 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11317.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSRVKFSRS ADAPAYQQGQ NQLYNELNLG RREEYDVLDK RRGRDPEMGG KPQRRKNPQE GLYNELQKDK MAEAYSEIGM KGERRRGKGH DGLYQGLSTA TKDTYDALHM QALPPR
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The CD247 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human CD247 General Information | |
|---|---|
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| Alternate Names | |
| T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, CD3Z, T3Z, TCRZ, CD3H, CD3Q, CD3-ZETA. | |
| Molecular Weight | |
| 15.4 kDa | |
| Chromosomal Location | |
| q24.2 on chromosome 1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD247 |
| Entrez Gene ID | 919 |
| UniProt ID(s) | P20963 |
| COSMIC ID Link(s) | CD247 |
| KEGG Gene ID(s) | hsa:919 |
| General Description of Recombinant Human CD247. | |
| Human CD247 produced in E. coli is a single polypeptide chain containing 136 amino acids (52-164) and having a molecular mass of 15.4kDa. CD247 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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