PDF DatasheetMouse CD274 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CD274 based upon sequence from Mouse
Host: QP11320 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CD274 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11320.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMFTITA PKDLYVVEYG SNVTMECRFP VERELDLLAL VVYWEKEDEQ VIQFVAGEED LKPQHSNFRG RASLPKDQLL KGNAALQITD VKLQDAGVYC CIISYGGADY KRITLKVNAP YRKINQRISV DPATSEHELI CQAEGYPEAE VIWTNSDHQPVSGKRSVTTS RTEGMLLNVT SSLRVNATAN DVFYCTFWRS QPGQNHTAEL IIPELPATHP PQNRTH
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: CD274 protein solution (1 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.15M NaCl.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Mouse CD274 General Information | |
|---|---|
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| Alternate Names | |
| Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1, A530045L16Rik. | |
| Molecular Weight | |
| 27.6 kDa | |
| Chromosomal Location | |
| C1 on chromosome 19 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Cd274 |
| Entrez Gene ID | 60533 |
| UniProt ID(s) | Q9EP73 |
| COSMIC ID Link(s) | Cd274 |
| KEGG Gene ID(s) | mmu:60533 |
| General Description of Recombinant Mouse CD274. | |
| Mouse CD274 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 246 amino acids (19-239 AA) and having a molecular mass of 27.6kDa (Molecular size on SDS-PAGE will appear higher). CD274 is fused to a 25 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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