PDF DatasheetHuman CD33 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CD33 based upon sequence from Human
Host: QP11327 protein expressed in E. Coli, Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CD33 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11327.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSDPNFWLQ VQESVTVQEG LCVLVPCTFF HPIPYYDKNS PVHGYWFREG AIISGDSPVA TNKLDQEVQE ETQGRFRLLG DPSRNNCSLS IVDARRRDNG SYFFRMERGS TKYSYKSPQL SVHVTDLTHR PKILIPGTLE PGHSKNLTCS VSWACEQGTP PIFSWLSAAP TSLGPRTTHS SVLIITPRPQ DHGTNLTCQV KFAGAGVTTE RTIQLNVTYV PQNPTTGIFP GDGSGKQETR AGVVH
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: CD33 protein solution (1 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea, and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human CD33 General Information | |
|---|---|
| Alternate Names | |
| CD33Molecule, CD33Antigen (Gp67), Sialic Acid Binding Ig-Like Lectin 3, Sialic Acid-Binding Ig-Like Lectin 3, SIGLEC3, gp67, SIGLEC-3, Myeloid Cell Surface AntigenCD33, p67, Siglec-3, CD33Antigen. | |
| Molecular Weight | |
| 29.1 kDa | |
| Chromosomal Location | |
| q13.41 on chromosome 19 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD33 |
| Entrez Gene ID | 945 |
| UniProt ID(s) | P20138 |
| COSMIC ID Link(s) | CD33 |
| KEGG Gene ID(s) | hsa:945 |
| General Description of Recombinant Human CD33. | |
| Human CD33 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 265 amino acids (18-259 AA) and having a molecular mass of 29.1kDa. CD33 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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