PDF DatasheetHuman CD3G Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CD3G based upon sequence from Human
Host: QP11332 protein expressed in E. Coli, Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CD3G was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11332.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSQSIKGNH LVKVYDYQED GSVLLTCDAE AKNITWFKDG KMIGFLTEDK KKWNLGSNAK DPRGMYQCKG SQNKSKPLQV YYRMCQNCIE LNAATIS
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: CD3G protein solution (1 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human CD3G General Information | |
|---|---|
| Alternate Names | |
| T-cell surface glycoprotein CD3 gamma chain, CD3-GAMMA, T3G, CD3G. | |
| Molecular Weight | |
| 13.1 kDa | |
| Chromosomal Location | |
| q23.3 on chromosome 11 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD3G |
| Entrez Gene ID | 917 |
| UniProt ID(s) | P09693 |
| COSMIC ID Link(s) | CD3G |
| KEGG Gene ID(s) | hsa:917 |
| General Description of Recombinant Human CD3G. | |
| Human CD3G produced in E. Coli is a single, non-glycosylated polypeptide chain containing 117 amino acids (23-116AA ) and having a molecular mass of 13.1kDa. CD3G is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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