PDF DatasheetHuman CDK1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CDK1 based upon sequence from Human
Host: QP11372 protein expressed in Insect, E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CDK1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11372.
Bioactivity Data: Untested
Amino Acid Sequence: MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR EISLLKELRH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM DSSLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR YSTPVDIWSI GTIFAELATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNT FPKWKPGSLA SHVKNLDENG LDLLSKMLIY DPAKRISGKM ALNHPYFNDL DNQIKKMLEH HHHHH
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: CDK1 protein solution (0.5 mg/ml) contains Phosphate buffered saline (pH 7.4), 30% glycerol, 2mM DTT and 0.1mM PMSF.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human CDK1 General Information | |
|---|---|
| Alternate Names | |
| Cyclin-Dependent Kinase 1, CDC2, Cell Division Cycle 2, G1 To S And G2 To M, Cell Division Control Protein 2 Homolog, Cell Division Protein Kinase 1, P34 Protein Kinase, P34CDC2, CDC28A, Cell Cycle Controller CDC2, EC 2.7. 11.22, EC 2.7. 11.23, CDKN1, Cyclin-dependent kinase 1. | |
| Molecular Weight | |
| 35.1 kDa | |
| Chromosomal Location | |
| q21.2 on chromosome 10 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CDK1 |
| Entrez Gene ID | 983 |
| UniProt ID(s) | P06493 |
| COSMIC ID Link(s) | CDK1 |
| KEGG Gene ID(s) | hsa:983 |
| General Description of Recombinant Human CDK1. | |
| CDK1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 305 amino acids (1-297AA ) and having a molecular mass of 35.1kDa. CDK1 is expressed with an 8 amino acid His tag at C-Terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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