PDF DatasheetHuman CDK3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CDK3 based upon sequence from Human
Host: QP11375 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CDK3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11375.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDMFQKV EKIGEGTYGV VYKAKNRETG QLVALKKIRL DLEMEGVPST AIREISLLKE LKHPNIVRLL DVVHNERKLY LVFEFLSQDL KKYMDSTPGS ELPLHLIKSY LFQLLQGVSF CHSHRVIHRD LKPQNLLINE LGAIKLADFG LARAFGVPLR TYTHEVVTLW YRAPEILLGS KFYTTAVDIW SIGCIFAEMV TRKALFPGDS EIDQLFRIFR MLGTPSEDTW PGVTQLPDYK GSFPKWTRKG LEEIVPNLEP EGRDLLMQLL QYDPSQRITA KTALAHPYFS SPEPSPAARQ YVLQRFRH
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The CDK3 solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1M Urea and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human CDK3 General Information | |
|---|---|
| Alternate Names | |
| Cyclin-dependent kinase 3, Cell division protein kinase 3, CDK3, CDKN3. | |
| Molecular Weight | |
| 37.4 kDa | |
| Chromosomal Location | |
| q25.1 on chromosome 17 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CDK3 |
| Entrez Gene ID | 1018 |
| UniProt ID(s) | Q00526 |
| COSMIC ID Link(s) | CDK3 |
| KEGG Gene ID(s) | hsa:1018 |
| General Description of Recombinant Human CDK3. | |
| Human CDK3 produced in E. coli is a single polypeptide chain containing 328 amino acids (1-305) and having a molecular mass of 37.4kDa. CDK3 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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