PDF DatasheetHuman CDKN1B Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CDKN1B based upon sequence from Human
Host: QP11377 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CDKN1B was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11377.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSNVRVSNGS PSLERMDARQ AEHPKPSACR NLFGPVDHEE LTRDLEKHCR DMEEASQRKW NFDFQNHKPL EGKYEWQEVE KGSLPEFYYR PPRPPKGACK VPAQESQDVS GSRPAAPLIG APANSEDTHL VDPKTDPSDS QTGLAEQCAG IRKRPATDDS STQNKRANRT EENVSDGSPN AGSVEQTPKK PGLRRRQT
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: CDKN1B protein solution (0.25 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human CDKN1B General Information | |
|---|---|
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| Alternate Names | |
| Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1, MEN4, CDKN4, MEN1B. | |
| Molecular Weight | |
| 24.2 kDa | |
| Chromosomal Location | |
| p13.1 on chromosome 12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CDKN1B |
| Entrez Gene ID | 1027 |
| UniProt ID(s) | P46527 |
| COSMIC ID Link(s) | CDKN1B |
| KEGG Gene ID(s) | hsa:1027 |
| General Description of Recombinant Human CDKN1B. | |
| Human CDKN1B produced in E. Coli is a single, non-glycosylated polypeptide chain containing 218 amino acids (1-198 AA ) and having a molecular mass of 24.2kDa (Molecular weight on SDS-PAGE will appear higher). CDKN1B is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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