PDF DatasheetHuman CHMP4A Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CHMP4A based upon sequence from Human
Host: QP11421 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CHMP4A was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11421.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSRRRPEDGL GKAGPCVMRH HPPRSKAEVW RTLRGGGGRG ELAMSGLGRL FGKGKKEKGP TPEEAIQKLK ETEKILIKKQ EFLEQKIQQE LQTAKKYGTK NKRAALQALR RKKRFEQQLA QTDGTLSTLE FQREAIENAT TNAEVLRTME LAAQSMKKAY QDMDIDKVDE LMTDITEQQE VAQQISDAIS RPMGFGDDVD EDELLEELEE LEQEELAQEL LNVGDKEEEP SVKLPSVPST HLPAGPAPKV DEDEEALKQL AEWVS
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The CHMP4A solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 0.1mMPMSF, 1mM EDTA, 2mM DTT and 50% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human CHMP4A General Information | |
|---|---|
| Alternate Names | |
| Charged multivesicular body protein 4A, C14orf123, HSPC134, VPS32A, Vacuolar protein sorting-associated protein 32-1, chromatin modifying protein 4A, SHAX2, SNF7-1, SNF7 homolog associated with Alix-2, chromosome 14 open reading frame 123, CHMP4B, CHMP4a. | |
| Molecular Weight | |
| 32.0 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9BY43 |
| General Description of Recombinant Human CHMP4A. | |
| Human CHMP4A produced in E. coli is a single, non-glycosylated polypeptide chain containing 285 amino acids (1-265 and having a molecular mass of 32.0kDa. CHMP4A is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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