PDF DatasheetCanine Clusterin Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Clusterin based upon sequence from Canine
Host: QP11455 protein expressed in E. Coli, HEK 293.
Available Tags: His, Flag Tag
Protein Construction: A cDNA sequence encoding the sequence of Clusterin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: Add deionized H2O to a working volume of 0.5 mg/ml and let the lyophilized pellet dissolve completely.
Bioactivity Data: Untested
Amino Acid Sequence: MKHHHHHHAS DQAVSDTELQ EMSTEGSKYI NKEIKNALKG VKQIKTLIEQ TNEERKSLLS NLEEAKKKKE DALNDTKDSE TKLKASQGVC NDTMMALWEE CKPCLKQTCM KFYARVCRSG SGLVGHQLEE FLNQSSPFYF WMNGDRIDSL LENDRQQTHA LDVMQDSFNR ASSIMDELFQ DRFFTREPQD TYHYSPFSLF QRRPFFNPKF RIARNIIPFP RFQPLNFHDM FQPFFDMIHQ AQQAMDVNLH RIPYHFPIEF PEEDNRTVCK EIRHNSTGCL KMKDQCEKCQ EILSVDCSSN NPAQVQLRQE LSNSLQIAEK FTKLYDELLQ SYQEKMFNTS SLLKQLNEQF SWVSQLANLT QSEDPFYLQV TTVGSQTSDS NVPVGFTKVV VKLFDSDPIT VMIPEAVSRN NPKFMETVAE KALQEYRQKHREE
Purity: Greater than 95% as determined by SDS PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Canine Clusterin was lyophilized from PBS, pH 7.5.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Canine Clusterin General Information | |
|---|---|
| Alternate Names | |
| CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Glycoprotein 80, Gp80, CLU, Clusterin, Apolipoprotein J, Apo-J. | |
| Molecular Weight | |
| 50.6 kDa | |
| Chromosomal Location | |
| on chromosome 25 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CLU |
| Entrez Gene ID | 442971 |
| UniProt ID(s) | P25473 |
| COSMIC ID Link(s) | CLU |
| KEGG Gene ID(s) | cfa:442971 |
| General Description of Recombinant Canine Clusterin. | |
| Apolipoprotein-J canine Recombinant produced in E. Coli is a single, non-glycosylated, Polypeptide chain containing 433 amino acids and having a molecular mass of 50.6 kDa. The protein is fused to His tag at N-Terminus. The Apolipoprotein-J canine is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. The amino acids sequence is identical to UniProtKB/Swiss-Prot entry P25473 amino acids 23445. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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