PDF DatasheetHuman Clusterin Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Clusterin based upon sequence from: Human
Host: QP8529 protein expressed in E. coli.
Tag: His
Protein Construction: A DNA sequence encoding the Homo sapiens (Human) Clusterin, was expressed in the hosts and tags indicated. Please select your host/tag option, above.
Application Notes: Please contact us for application specific information for QP8529.
Bioactivity Data: Untested
Full Length? Partial (see sequence information for more details).
Expression Region: Asp23 – Arg224
Amino Acid Sequence: DQTVSDNELQ EMSNQGSKYV NKEIQNAVNG VKQIKTLIEK TNEERKTLLS NLEEAKKKKE DALNETRESE TKLKELPGVC NETMMALWEE CKPCLKQTCM KFYARVCRSG SGLVGRQLEE FLNQSSPFYF WMNGDRIDSL LENDRQQTHM LDVMQDHFSR ASSIIDELFQ DRFFTREPQD TYHYLPFSLP HRRPHFFFPK SR
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions:
Concentration of Human Clusterin Protein:
Endotoxin Levels: Not determined.
Buffer: Tris-based buffer, 50% glycerol
Storage Conditions: Store at -20C to -80C.
| Recombinant Human Clusterin Protein General Information | |
|---|---|
| Alternate Names | |
| AAG4; NA1/NA2; APO-J; SP-40; CLU1; KUB1; TRPM-2; TRPM2; APOJ; CLU2; SGP-2; CLI; SGP2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | APOJ |
| Entrez Gene ID | 1191 |
| Ensemble Gene ID | ENSG00000120885 |
| RefSeq Protein Accession(s) | NP_001822.3 |
| RefSeq mRNA Accession(s) | NM_001831.3, XM_006716284.2 |
| UniProt ID(s) | P10909 |
| UniGene ID(s) | Hs.436657 |
| HGNC ID(s) | HGNC:2095 |
| KEGG Gene ID(s) | hsa:1191 |
| PharmGKB ID(s) | PA26620 |
| General Description of Recombinant Human Clusterin Protein. | |
| Isoform 1 functions as Extracellular domain chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complent. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation | |
Limitations and Performance Guarantee
This is a life science research product (for Research Use Only). This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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