PDF DatasheetHuman CYP2E1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CYP2E1 based upon sequence from Human
Host: QP11584 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CYP2E1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11584.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSWNLPPG PFPLPIIGNL FQLELKNIPK SFTRLAQRFG PVFTLYVGSQ RMVVMHGYKA VKEALLDYKD EFSGRGDLPA FHAHRDRGII FNNGPTWKDI RRFSLTTLRN YGMGKQGNES RIQREAHFLL EALRKTQGQP FDPTFLIGCA PCNVIADILF RKHFDYNDEK FLRLMYLFNE NFHLLSTPWL QLYNNFPSFL HYLPGSHRKV IKNVAEVKEY VSERVKEHHQ SLDPNCPRDL TDCLLVEMEK EKHSAERLYT MDGITVTVAD LFFAGTETTS TTLRYGLLIL MKYPEIEEKL HEEIDRVIGP SRIPAIKDRQ EMPYMDAVVH EIQRFITLVP SNLPHEATRD TIFRGYLIPK GTVVVPTLDS VLYDNQEFPD PEKFKPEHFL NENGKFKYSD YFKPFSTGKR VCAGEGLARM ELFLLLCAIL QHFNLKPLVD PKDIDLSPIH IGFGCIPPRY KLCVIPRS
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: CYP2E1 protein solution (0.5 mg/ml) containing 20mM Tris-HCl (pH 8.0) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human CYP2E1 General Information | |
|---|---|
| Alternate Names | |
| Cytochrome P450, Family 2, Subfamily E, Polypeptide 1, CYP2E, Cytochrome P450, Subfamily IIE (Ethanol-Inducible), Polypeptide 1, 4-Nitrophenol 2-Hydroxylase, Cytochrome P450-J, P450C2E, CYPIIE1, Flavoprotein-Linked Monooxygenase, Microsomal Monooxygenase, Xenobiotic Monooxygenase, Cytochrome P450 2E1, EC 1.14.13. n7, EC 1.14.14.1, EC 1.14.13. -, P450-J, CPE1, Cytochrome P450 2E1. | |
| Molecular Weight | |
| 56.2 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P05181 |
| General Description of Recombinant Human CYP2E1. | |
| Human CYP2E1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 488 amino acids (29-493 AA) and having a molecular mass of 56.2kDa. CYP2E1 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used for any other purpose. This product is guaranteed to work for a period of one year when stored at -70°C or colder.
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