PDF DatasheetHuman DBR1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant DBR1 based upon sequence from Human
Host: QP11598 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of DBR1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11598.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHVKFAAL MQHQAKDKGQ TARATKFLAL DKCLPHRDFL QILEIEHDPS APDYLEYDIE WLTILRATDD LINVTGRLWN MPENNGLHAR WDYSATEEGM KEVLEKLNHD LKVPCNFSVT AACYDPSKPQ TQMQLIHRIN PQTTEFCAQL GIIDINVRLQ KSKEEHHVCG EYEEQDDVES NDSGEDQSEY NTDTSALSSI NPDEIMLDEE EDEDSIVSAH SGMNTPSVEP SDQASEFSAS FSDVRILPGS MIVSSDDTVD STIDREGKPG GTVESGNGED LTKVPLKRLS DEHEPEQRKK IKRRNQAIYA AVDDDDDDAA
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The DBR1 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human DBR1 General Information | |
|---|---|
| Alternate Names | |
| Debranching RNA Lariats 1, Lariat Debranching Enzyme, Debranching Enzyme Homolog 1, RNA Lariat Debranching Enzyme, EC 3.1. -. -. | |
| Molecular Weight | |
| 38 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9UK59 |
| General Description of Recombinant Human DBR1. | |
| Human DBR1 produced in E. coli is a single, non-glycosylated polypeptide chain containing 340 amino acids (228-544) and having a molecular mass of 38kDa (molecular size on SDS-PAGE will appear higher). DBR1 is fused to a 23 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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