PDF DatasheetHuman DCT Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant DCT based upon sequence from Human
Host: QP11601 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of DCT was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11601.
Bioactivity Data: Untested
Amino Acid Sequence: QFPRVCMTVD SLVNKECCPR LGAESANVCG SQQGRGQCTE VRADTRPWSG PYILRNQDDR ELWPRKFFHR TCKCTGNFAG YNCGDCKFGW TGPNCERKKP PVIRQNIHSL SPQEREQFLG ALDLAKKRVH PDYVITTQHW LGLLGPNGTQ PQFANCSVYD FFVWLHYYSV RDTLLGPGRP YRAIDFSHQG PAFVTWHRYH LLCLERDLQR LIGNESFALP YWNFATGRNE CDVCTDQLFG AARPDDPTLI SRNSRFSSWE TVCDSLDDYN HLVTLCNGTY EGLLRRNQMG RNSMKLPTLK DIRDCLSLQK FDNPPFFQNS TFSFRNALEG FDKADGTLDS QVMSLHNLVH SFLNGTNALP HSAANDPIFV VLHSFTDAIF DEWMKRFNPP ADAWPQELAP IGHNRMYNMV PFFPPVTNEE LFLTSDQLGY SYAIDLPVSV EETPGWPTTH HHHHH
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: STK3 protein solution (0.25 mg/ml) containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human DCT General Information | |
|---|---|
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| Alternate Names | |
| L-dopachrome tautomerase (EC:5.3. 3.12), DCT, DT, L-dopachrome Delta-isomerase Tyrosinase-related protein 2, TRP-2, TRP2, TYRP2. | |
| Molecular Weight | |
| 52.1 kDa | |
| Chromosomal Location | |
| q32.1 on chromosome 13 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | DCT |
| Entrez Gene ID | 1638 |
| UniProt ID(s) | P40126 |
| COSMIC ID Link(s) | DCT |
| KEGG Gene ID(s) | hsa:1638 |
| General Description of Recombinant Human DCT. | |
| Human DCT produced in Sf9 Insect cell is a single, non-glycosylated polypeptide chain containing 455 amino acids (24-472aAA) and having a molecular mass of 52.1kDa (Migrates at 50-70kDa on SDS-PAGE under reducing conditions). DCT is fused to a 6 amino acid His tag at C-Terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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