PDF DatasheetHuman DDIT4 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant DDIT4 based upon sequence from Human
Host: QP11609 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of DDIT4 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11609.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF LPGFSQSLTL STGFRVIKKK LYSSEQLLIE EC
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The DDIT4 protein solution (0.25 mg/1ml) is formulated in 20 mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA and 30% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human DDIT4 General Information | |
|---|---|
| Alternate Names | |
| DNA-damage-inducible transcript 4, RTP801, REDD-1, Dig2, FLJ20500, Protein regulated in development and DNA damage response 1, HIF-1 responsive protein RTP801, RP11-442H21.1. | |
| Molecular Weight | |
| 27.5 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9NX09 |
| General Description of Recombinant Human DDIT4. | |
| DDIT4 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (1-232. AA) and having a molecular mass of 27.5kDa. DDIT4 is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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