PDF DatasheetHuman DDT Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant DDT based upon sequence from Human
Host: QP11612 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of DDT was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11612.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: DDT Human solution containing 20mM Tris-HCl pH 8.0, & 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human DDT General Information | |
|---|---|
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| Alternate Names | |
| EC 4.1. 1.84, DDCT, D-dopachtome decarboxylase, D-Dopachrome Tautomerase. | |
| Molecular Weight | |
| 14.8 kDa | |
| Chromosomal Location | |
| q11.23 on chromosome 22 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | DDT |
| Entrez Gene ID | 1652 |
| UniProt ID(s) | P30046 |
| COSMIC ID Link(s) | DDT |
| KEGG Gene ID(s) | hsa:1652 |
| General Description of Recombinant Human DDT. | |
| DDT Recombinant E. coli produced in E. Coli is a single, non-glycosylated polypeptide chain containing 138 amino acids (1-118 AA ) and having a molecular mass of 14.8 kDa. The DDT is fused to a 20 amino acids His-Tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used for any other purpose. This product is guaranteed to work for a period of one year when stored at -70°C or colder.
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