PDF DatasheetHuman DKK3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant DKK3 based upon sequence from Human
Host: QP11668 protein expressed in E. Coli, HEK 293, Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of DKK3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11668.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHYHH SSGLVPRGSH MGSMAPAPTA TSAPVKPGPA LSYPQEEATL NEMFREVEEL MEDTQHKLRS AVEEMEAEEA AAKASSEVNL ANLPPSYHNE TNTDTKVGNN TIHVHREIHK ITNNQTGQMV FSETVITSVG DEEGRRSHEC IIDEDCGPSM YCQFASFQYT CQPCRGQRML CTRDSECCGD QLCVWGHCTK MATRGSNGTI CDNQRDCQPG LCCAFQRGLL FPVCTPLPVE GELCHDPASR LLDLITWELE PDGALDRCPC ASGLLCQPHS HSLVYVCKPT FVGSRDQDGE ILLPREVPDE YEVGSFMEEV RQELEDLERS LTEEMALREP AAAAAALLGG EEI
Purity: Greater than 85% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The DKK3 solution (0.5 mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 1M Urea, 1mM DTT and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human DKK3 General Information | |
|---|---|
| Alternate Names | |
| Dickkopf 3 homolog (Xenopus laevis), dickkopf-related protein 3, regulated in glioma, RIG, RIG-like 7-1, RIG-like 5-6, Dkk-3, REIC. | |
| Molecular Weight | |
| 38.8 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9UBP4 |
| General Description of Recombinant Human DKK3. | |
| Human DKK3 produced in E. coli is a single polypeptide chain containing 353 amino acids (22-350) and having a molecular mass of 38.8 kDa. DKK3 is fused to a 24 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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