PDF DatasheetHuman DNMT3L Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant DNMT3L based upon sequence from Human
Host: QP11694 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of DNMT3L was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11694.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFMAAIP ALDPEAEPSM DVILVGSSEL SSSVSPGTGR DLIAYEVKAN QRNIEDICIC CGSLQVHTQH PLFEGGICAP CKDKFLDALF LYDDDGYQSY CSICCSGETL LICGNPDCTR CYCFECVDSL VGPGTSGKVH AMSNWVCYLC LPSSRSGLLQ RRRKWRSQLK AFYDRESENP LEMFETVPVW RRQPVRVLSL FEDIKKELTS LGFLESGSDP GQLKHVVDVT DTVRKDVEEW GPFDLVYGAT PPLGHTCDRP PSWYLFQFHR LLQYARPKPG SPRPFFWMFV DNLVLNKEDL DVASRFLEME PVTIPDVHGG SLQNAVRVWS NIPAIRSRHW ALVSEEELSL LAQNKQSSKL AAKWPTKLVK NCFLPLREYF KYFSTELTSS L
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The DNMT3L solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human DNMT3L General Information | |
|---|---|
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| Alternate Names | |
| DNMT3L, DNA (Cytosine-5-)-Methyltransferase 3-Like, Human Cytosine-5-Methyltransferase 3-Like Protein 11, Cytosine-5-Methyltransferase 3-Like Protein, DNA (Cytosine-5)-Methyltransferase 3-Like. | |
| Molecular Weight | |
| 46.2 kDa | |
| Chromosomal Location | |
| q22.3 on chromosome 21 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | DNMT3L |
| Entrez Gene ID | 29947 |
| UniProt ID(s) | Q9UJW3 |
| COSMIC ID Link(s) | DNMT3L |
| KEGG Gene ID(s) | hsa:29947 |
| General Description of Recombinant Human DNMT3L. | |
| Human DNMT3L produced in E. coli is a single, non-glycosylated polypeptide chain containing 411 amino acids (1-386) and having a molecular mass of 46.2kDa. DNMT3L is fused to a 25 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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