PDF DatasheetHuman DYRK1A Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant DYRK1A based upon sequence from Human
Host: QP11722 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of DYRK1A was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11722.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH RSYEIDSLIG KGSFGQVVKA YDRVEQEWVA IKIIKNKKAF LNQAQIEVRL LELMNKHDTE MKYYIVHLKR HFMFRNHLCL VFEMLSYNLY DLLRNTNFRG VSLNLTRKFA QQMCTALLFL ATPELSIIHC DLKPENILLC NPKRSAIKIV DFGSSCQLGQ RIYQYIQSRF YRSPEVLLGM PYDLAIDMWS LGCILVEMHT GEPLFSGANE VDQMNKIVEV LGIPPAHILD QAPKARKFFE KLPDGTWSLK KTKDGKREYK PPGTRKLHNI LGVETGGPGG RRAGESGHTV ADYLKFKDLI LRMLDYDPKT RIQPYYALQH SFF
Purity: Greater than 80% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The DYRK1A solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered colorless solution.
| Recombinant Human DYRK1A General Information | |
|---|---|
| Alternate Names | |
| Dual Specificity Yak1-related kinase, Dyrk; PSK47, Dual specificity tyrosine-phosphorylation-regulated kinase 1A, Dual specificity YAK1-related kinase, Protein kinase minibrain homolog, RP86, Dyrk1a, MNBH. | |
| Molecular Weight | |
| 39.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q63470 |
| General Description of Recombinant Human DYRK1A. | |
| Human DYRK1A produced in E. coli is a single, non-glycosylated polypeptide chain containing 343 amino acids (159-479AA) and having a molecular mass of 39.4kDa. DYRK1A is fused to a 22 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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