PDF DatasheetHuman ECHS1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ECHS1 based upon sequence from Human
Host: QP11739 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of ECHS1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11739.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The ECHS1 protein solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8), 1mM DTT,0.1M NaCl and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human ECHS1 General Information | |
|---|---|
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| Alternate Names | |
| Enoyl-CoA hydratase 1, SCEH. | |
| Molecular Weight | |
| 30.6 kDa | |
| Chromosomal Location | |
| q26.3 on chromosome 10 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ECHS1 |
| Entrez Gene ID | 1892 |
| UniProt ID(s) | P30084 |
| COSMIC ID Link(s) | ECHS1 |
| KEGG Gene ID(s) | hsa:1892 |
| General Description of Recombinant Human ECHS1. | |
| Human ECHS1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 284 amino acids (28-290 AA ) and having a molecular mass of 30.6kDa. ECHS1 is fused to a 21 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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