PDF DatasheetE. Coli Ecotin Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Ecotin based upon sequence from E. Coli
Host: QP11741 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of Ecotin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11741.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAESVQPLEK IAPYPQAEKG MKRQVIQLTP QEDESTLKVE LLIGQTLEVD CNLHRLGGKL ENKTLEGWGY DYYVFDKVSS PVSTMMACPD GKKEKKFVTA YLGDAGMLRY NSKLPIVVYT PDNVDVKYRV WKAEEKIDNA VVR
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The Ecotin protein solution (1 mg/ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 50mM NaCl and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant E. Coli Ecotin General Information | |
|---|---|
| Alternate Names | |
| E. coli serine protease inhibitor. | |
| Molecular Weight | |
| 18.3 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P23827 |
| General Description of Recombinant E. Coli Ecotin. | |
| Ecotin produced in E. Coli is a single, non-glycosylated polypeptide chain containing 163 amino acids (21-162AA ) and having a molecular mass of 18.3kDa. Ecotin is fused to a 20 amino acid His-tag at N-terminus & purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bios products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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