PDF DatasheetHuman EDF1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant EDF1 based upon sequence from Human
Host: QP11745 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of EDF1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11745.
Bioactivity Data: Untested
Amino Acid Sequence: MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK QHSITKNTAK LDRETEELHH DRVTLEVGKV IQQGRQSKGL TQKDLATKIN EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPRAKLE HHHHHH
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: 0.5 mg/ml solution containing 20mM Tris pH 8.0, 1mM DTT, 0.1M NaCl & 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human EDF1 General Information | |
|---|---|
| Alternate Names | |
| EDF-1, MBF1, Multiprotein-bridging factor 1. | |
| Molecular Weight | |
| 17.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | O60869 |
| General Description of Recombinant Human EDF1. | |
| Human EDF1 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 156 amino acids (1-148 AA ) and having a molecular mass of 17.4 kDa. The EDF1 is fused to 8 amino acid His-Tag at C-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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