PDF DatasheetHuman EDN2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant EDN2 based upon sequence from Human
Host: QP11746 protein expressed in E. Coli, |||.
Available Tags: His, Untagged
Protein Construction: A cDNA sequence encoding the sequence of EDN2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes: Please contact us for application specific information for QP11746.
Bioactivity Data: Untested
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSQAAATLE QPASSSHAQG THLRLRRCSC SSWLDKECVY FCHLDIIWVN TPEQTAPYGL GNPPRRRRRS LPRRCQCSSA RDPACATFCL RRPWTEAGAV PSRKSPADVF QTGKTGATTG ELLQRLRDIS TVKSLFAKRQ QEAMREPRST HSRWRKR
Purity: Greater than 85.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: EDN2 protein solution (1 mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered colorless solution.
| Recombinant Human EDN2 General Information | |
|---|---|
| Alternate Names | |
| ET2, PPET2, Endothelin-2, ET-2, Preproendothelin-2, EDN2. | |
| Molecular Weight | |
| 19.9 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P20800 |
| General Description of Recombinant Human EDN2. | |
| Human EDN2 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 177 amino acids (25-178 AA ) and having a molecular mass of 19.9kDa. EDN2 is fused to a 23 amino acid His-tag at N-terminus. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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